Tag: activated cell sorting

  • The Y protects against food related diseases by sustaining thermogenic fat

    The Y protects against food related diseases by sustaining thermogenic fat

    The following antibodies were used for fluorescent activated cell sorting (FACS) and flow-through. AF700 anti-CD45, rabbit anti-NPY, mouse anti-H2A.X, mouse anti-histone H3 and mouse anti- ChK1 were used for FACS and flow- through experiments respectively. Cells collected following in vitro experiments were washed once with PBS and fixed using 4% PFA for at least 4…